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Image Search Results


Representative fluorescent images (each image is an overlay with DAPI nuclear staining) and quantitative CTCF values for HSP70, GRP78, p‐IRE1α, p‐PERK, p‐eIF2α, and ATF6 in GC1 cells across all groups (C, SAL, HSM, HSMSAL). Composite images were generated by merging individual fluorescence channels. Data are expressed as mean ± SD ( n = 3, 10 fields/group). Statistical analysis: one‐way ANOVA, followed by Sidak's post hoc test. (* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001; statistical analysis was performed using graphpad Software). Scale bar: 50 μm.

Journal: FEBS Open Bio

Article Title: The effect of salubrinal on the endoplasmic reticulum stress pathway in heat‐stressed spermatogonial cells in vitro

doi: 10.1002/2211-5463.70169

Figure Lengend Snippet: Representative fluorescent images (each image is an overlay with DAPI nuclear staining) and quantitative CTCF values for HSP70, GRP78, p‐IRE1α, p‐PERK, p‐eIF2α, and ATF6 in GC1 cells across all groups (C, SAL, HSM, HSMSAL). Composite images were generated by merging individual fluorescence channels. Data are expressed as mean ± SD ( n = 3, 10 fields/group). Statistical analysis: one‐way ANOVA, followed by Sidak's post hoc test. (* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001; statistical analysis was performed using graphpad Software). Scale bar: 50 μm.

Article Snippet: After that, cells were incubated with blocking serum for 30 min. HSP70 (Biorbyt, orb157591, 1 : 200), p‐eIF2α (Bioss, BS‐4842, 1 : 200), p‐PERK (Bioss, bs‐3330R, 1 : 200), p‐IRE1α (Biorbyt, orb157704, 1 : 200), ATF6 (Bioss, bs‐1634R, 1 : 200), and GRP78 (Bioss, bs‐1219R, 1 : 200) rabbit polyclonal primary antibodies were incubated overnight for approximately 16 h at 4 °C in a humidified chamber.

Techniques: Staining, Generated, Fluorescence, Software

Representative fluorescent images (each image is an overlay with DAPI nuclear staining) and quantitative CTCF values for HSP70, GRP78, p‐IRE1α, p‐PERK, p‐eIF2α, and ATF6 in GC2 cells across all groups (C, SAL, HSM, HSMSAL). Composite images were generated by merging individual fluorescence channels. Data are expressed as mean ± SD ( n = 3, 10 fields per group). Statistical analysis: one‐way ANOVA, followed by Sidak's post hoc test. (* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001; statistical analysis was performed using graphpad Software). Scale bar: 50 μm.

Journal: FEBS Open Bio

Article Title: The effect of salubrinal on the endoplasmic reticulum stress pathway in heat‐stressed spermatogonial cells in vitro

doi: 10.1002/2211-5463.70169

Figure Lengend Snippet: Representative fluorescent images (each image is an overlay with DAPI nuclear staining) and quantitative CTCF values for HSP70, GRP78, p‐IRE1α, p‐PERK, p‐eIF2α, and ATF6 in GC2 cells across all groups (C, SAL, HSM, HSMSAL). Composite images were generated by merging individual fluorescence channels. Data are expressed as mean ± SD ( n = 3, 10 fields per group). Statistical analysis: one‐way ANOVA, followed by Sidak's post hoc test. (* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001; statistical analysis was performed using graphpad Software). Scale bar: 50 μm.

Article Snippet: After that, cells were incubated with blocking serum for 30 min. HSP70 (Biorbyt, orb157591, 1 : 200), p‐eIF2α (Bioss, BS‐4842, 1 : 200), p‐PERK (Bioss, bs‐3330R, 1 : 200), p‐IRE1α (Biorbyt, orb157704, 1 : 200), ATF6 (Bioss, bs‐1634R, 1 : 200), and GRP78 (Bioss, bs‐1219R, 1 : 200) rabbit polyclonal primary antibodies were incubated overnight for approximately 16 h at 4 °C in a humidified chamber.

Techniques: Staining, Generated, Fluorescence, Software